抗Human/Mouse/Rat GAPDH抗体(Anti-Human/Mouse/Rat GAPDH antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant human GAPDH/G3PDHMet1-Ala150Accession # P04406
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human, mouse, and rat GAPDH/G3PDH in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human GAPDH-2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Immunocytochemistry	5-25 µg/mL	See below

Western Blot
	Detection of Human/Mouse/Rat GAPDH/G3PDH by Western Blot. Western blot shows lysates of mouse, human, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat GAPDH/G3PDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GAPDH/G3PDH at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Immunocytochemistry
	GAPDH in HeLa Human Cell Line. GAPDH was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat GAPDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
	GAPDH/G3PDH in Human Kidney. GAPDH/G3PDH was detected in immersion fixed paraffin-embedded sections of normal human kidney using Goat Anti-Human/Mouse/Rat GAPDH/G3PDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei of convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.This application has not been tested in mouse or rat samples.
Immunohistochemistry
	GAPDH in Human Prostate Cancer Tissue. GAPDH was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human/Mouse/Rat GAPDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Simple Western
	Detection of Human GAPDH by Simple WesternTM. Simple Western lane view shows lysates of human brain (motor cortex) tissue and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for GAPDH at approximately 43 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat GAPDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Long Name	Glyceraldehyde-3-phosphate Dehydrogenase
Background	GAPDH
background_content	Background: GAPDH Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a 36‑40 kDa member of the GAPDH family of enzymes. It is a widely expressed heterotetramer that is found in both the nucleus and cytoplasm. Although GAPDH was initially identified as a glycolytic enzyme that converted G3P into 1,3 diphosphoglycerate, it is now recognized to participate in no less than endocytosis, membrane fusion, vesicular secretory transport, DNA replication and repair, and apoptosis. Human GAPDH is 335 amino acids (aa) in length and contains two NAD binding sites (Asp35 and Asn316) with a catalytic region between aa 151‑155. GAPDH contains more than 19 posttranslational modifications, including methylation, deamidation and phosphorylation. One splice variant shows a 10 aa substitution for aa 319‑335. Over amino acid 1‑150, human GAPDH shares 92% aa identity with mouse GAPDH.

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Citations

1. Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells
   Authors: CT Yang, R Ma, RA Axton, M Jackson, AH Taylor, A Fidanza, L Marenah, J Frayne, JC Mountford, LM Forrester
   Stem Cells, 2017;35(4):886-897.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. Salmonella Typhimurium disrupts Sirt1/AMPK checkpoint control of mTOR to impair autophagy
   Authors: R Ganesan, NJ Hos, S Gutierrez, J Fischer, JM Stepek, E Daglidu, M Krönke, N Robinson
   PLoS Pathog, 2017;13(2):e1006227.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells
   Authors: X Li, J Lu, Q Kan, X Li, Q Fan, Y Li, R Huang, A Slipicevic, HP Dong, L Eide, J Wang, H Zhang, V Berge, MA Goscinski, G Kvalheim, JM Nesland, Z Suo
   Sci Rep, 2017;7(1):5081.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Alterations in Adiposity and Glucose Homeostasis in Adult Gasp-1 Overexpressing Mice
   Authors: L Périè, A Parenté, F Baraige, L Magnol, V Blanquet
   Cell. Physiol. Biochem., 2017;44(5):1896-1911.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


5. Caspase-3/-7-Specific Metabolic Precursor for Bioorthogonal Tracking of Tumor Apoptosis
   Authors: MK Shim, HY Yoon, S Lee, MK Jo, J Park, JH Kim, SY Jeong, IC Kwon, K Kim
   Sci Rep, 2017;7(1):16635.
   Species: Human
   Sample Type: Whole Cells
   Application: WB


6. Up-regulation of VEGF by retinoic acid during hyperoxia prevents retinal neovascularization and retinopathy.
   Authors: Wang L, Shi P, Xu Z, Li J, Xie Y, Mitton K, Drenser K, Yan Q
   Invest Ophthalmol Vis Sci, 2014;55(7):4276-87.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


7. Prenatal retinoid deficiency leads to airway hyperresponsiveness in adult mice.
   Authors: Chen, Felicia, Marquez, Hector, Kim, Youn-Kyu, Qian, Jun, Shao, Fengzhi, Fine, Alan, Cruikshank, William, Quadro, Loredana, Cardoso, Wellingt
   J Clin Invest, 2014;124(2):801-11.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: WB


8. Effects of direct Renin inhibition on myocardial fibrosis and cardiac fibroblast function.
   Authors: Zhi, Hui, Luptak, Ivan, Alreja, Gaurav, Shi, Jianru, Guan, Jian, Metes-Kosik, Nicole, Joseph, Jacob
   PLoS ONE, 2013;8(12):e81612.
   Species: Rat
   Sample Type: Cell Lysates
   Application: WB


9. The hypoxic microenvironment upgrades stem-like properties of ovarian cancer cells.
   Authors: Liang D, Ma Y, Liu J, Trope CG, Holm R, Nesland JM, Suo Z
   BMC Cancer, 2012;12(0):201.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


10. Overexpression of carbonic anhydrase and HIF-1alpha in Wilms tumours.
    Authors: Dungwa JV, Hunt LP, Ramani P
    BMC Cancer, 2011;11(0):390.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB

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