抗Cas9 [7A9]抗体 | Anti-Cas9 [7A9] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

IP analysis was performed with lysate from HEK293T cells expressing Flag-tagged S. pyogenes Cas9 and either Cas9 antibody [7A9] followed by incubation with a 1:1 mixture of protein A/G sepharose beads (Cas9 Sup) or Cas9 antibody [7A9] crosslinked with a 1:1 mixture of protein A/G sepharose beads (Cas9 X-link). The precipitates were detected by the same antibody. Asterisk indicates IgG heavy chain.

ICC/IF analysis of HeLa cells transiently transfected with flag-tagged Cas9 using Cas9 antibody [7A9] at a dilution of 0.5 μg/ml (green) and DAPI (blue).

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	7A9
Isotype	IgG1
Application	WB, ICC/IF, IP

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APPLICATION

Application Note

Western Blotting Analysis: 0.5 μg/mL from a representative lot detected Cas9 in 1 μg of Myc-Cas9 expressing HEK293 cell lysate. Immunoprecipitation Analysis: A representative lot immunoprecipitated Cas9 in Flag-Cas9 and Myc-Cas9 expressing cell lysate (performed by an independent laboratory).

Specificity/Sensitivity	This antibody recognises both Cas9 and dCas9 (nuclease deficient Cas9)

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PROPERTIES

Form	Liquid
Buffer	Buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Immunogen	N-Terminal domain of Cas9.
Purification	Protein G purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Shen CC et al. Nucleic Acids Res 2018; (Epub) Synthetic switch to minimize CRISPR off-target effects by self-restricting Cas9 transcription and translation.	Application : WB
Germano G et al. Nature 2017; 552 (7683) : 116-120 Inactivation of DNA repair triggers neoantigen generation and impairs tumour growth.	Application : WB
Guo J et al. Protein Cell 2017; (Epub) An inducible CRISPR-ON system for controllable gene activation in human pluripotent stem cells.	Application : WB

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