抗MAP2 [AP-20]抗体 | Anti-MAP2 [AP-20] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	WB analysis of rat brain extract using GTX11268 MAP2 antibody[AP-20]. Lane A : Antibody dilution 1:500 Lane B : Secondary antibody only
	ICC/IF analysis of SH-SY-5Y cells using GTX11268 MAP2 antibody [AP-20] at 1:500(red) with DAPI(blue).Cells were fixed and permeabilized with methanol followed by methanol:acetone.
	ICC/IF analysis of B35 cells using GTX11268 MAP2 antibody [AP-20] at 1:100(red) with DAPI(blue).Cells were fixed and permeabilized with methanol followed by methanol:acetone.

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	AP-20
Isotype	IgG1
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat, Bovine, Quail, Xenopus, aquatic salamander

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500
ICC/IF	Assay dependent
IHC-P	Assay dependent

Not tested in other applications.

Positive Control	rat cerebral cortex

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PROPERTIES

Form	Liquid
Buffer	Ascites, 15 mM sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species	Bovine
Immunogen	bovine MAP2
Purification	Unpurified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	microtubule associated protein 2
Background	MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin, and other elements of the cytoskeleton.
Database	・ Gene ID: 281294 MAP2

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Berenice M?rquez-Valadez et al. Front. Neurosci. 2019; (Epub) The Systemic Administration of the Histamine H1 Receptor Antagonist/Inverse Agonist.	Application : IHC-P Reactivity : Rat
Tian E et al. Cell Rep 2016; 16 (3) : 781-92 Small-Molecule-Based Lineage Reprogramming Creates Functional Astrocytes.	Application : ICC/IF Reactivity : Mouse

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