抗ABAT, Mouse-Mono(UMAB178) UltraMAB抗体 | Anti-ABAT, Mouse-Mono(UMAB178) UltraMAB Antibody
掲載日情報:2015/11/06 現在Webページ番号:178036
OriGene社の抗ABAT, Mouse-Mono(UMAB178) UltraMAB抗体です。
本製品は、UltraMAB抗体シリーズ(三次元構造を有する組換え体ヒトタンパク質の全長を抗原として作製したモノクローナル抗体)です。
※本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 :2024年05月18日 08時55分現在]
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[在庫・価格 :2024年05月18日 08時55分現在]
Anti-ABAT, Mouse-Mono(UMAB178), UltraMAB
文献数: 0
- 商品コード:UM800070
- メーカー:ORI
- 包装:100μl
- 価格:¥185,000
- 在庫:無(未発注)
- 納期:3~4週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
クローン:UMAB178 Genbank No: 18 Gene Accession No: NM_020686 |
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法規制等 | |||
保存条件 | 法規備考 | ||
抗原種 | 免疫動物 | Mouse | |
交差性 | Human/Mouse/Rat | 適用 | IF,IHC,IP,Western Blot |
標識 | Unlabeled | 性状 | Affinity Purified |
吸収処理 | クラス | IgG | |
クロナリティ | Monoclonal | フォーマット | |
掲載カタログ |
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製品記事 |
UltraMAB抗体 |
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関連記事 |
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UltraMAB抗体について
OriGene社製品の中でも、特にUltraMAB抗体シリーズは、ヒト組織の免疫染色(IHC)に適した非常に特異性の高いモノクローナル抗体です。抗原にはTrueMAB抗体と同様、組換え体ヒト全長タンパク質を使用しています。1万種類を超えるタンパク質をスポットしたマイクロアレイを用い、標的タンパク質以外の分子との交差反応がないことを確認済みの、癌研究・病理学・解剖学向けの製品です。
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Product Data
Immunogen | Human recombinant protein fragment corresponding to amino acids 29-323 of human ABAT(NP_065737) produced in E.coli. | ||
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Clone Name | Clone UMAB178 | Isotype | IgG1 |
Species Reactivity | Human | Concentration | 0.5~1.0 mg/ml (Lot Dependent) |
Guaranteed Application | WB, IHC, IF, 10K-CHIP | Suggested Dilutions | IHC 1:100~200, |
Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. | ||
Purification | Purified from cell culture supernatant by affinity chromatography |
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Reference Data
Target Name | Homo sapiens 4-aminobutyrate aminotransferase (ABAT), transcript variant 1 | ||
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Alternative Name | GABA-AT; GABAT; NPD009 | ||
Database Link | NP_065737 | ||
Function | 4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq, Jul 2008]. | ||
Related Pathway |
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Image
Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). | |
Western blot analysis of extracts (15ug) from 9 Human tissue by using anti-ABAT monoclonal antibody (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: colon:;9:Spleen).(1:500) Dilution: 1:500 | |
Immunohistochemical staining of paraffin-embedded carcinoma of human kidney tissue using ABAT clone UMAB178, mouse monoclonal antibody. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800070 was diluted 1:200 using HRP detection and DAB chromogen. Images shows strong cytoplasmic and membranous staining is present in the tumor cells. | |
Immunohistochemical staining of paraffin-embedded carcinoma of human liver tissue using ABAT clone UMAB178, mouse monoclonal antibody. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800070 was diluted 1:200 using HRP detection and DAB chromogen. Images shows strong cytoplasmic and membranous staining is present in the tumor cells. | |
Immunohistochemical staining of paraffin-embedded human pancreas tissue using ABAT clone UMAB178, mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, UM800070was diluted 1:200 and detection shown with HRP enzyme and DAB chromogen. Images shows strong cytoplasmic and membranous staining is present in the exocrine gladular cells. | |
Immunohistochemical staining of paraffin-embedded carcinoma of human pancreas tissue using ABAT clone UMAB178, mouse monoclonal antibody. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800070 was diluted 1:200 and detection shown with HRP enzyme and DAB chromogen. Images shows strong cytoplasmic and membranous staining is present in the tumor cells. | |
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ABAT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800070)(1:200) | |
Immunohistochemical staining of paraffin-embedded human liver using ABAT clone UMAB178, mouse monoclonal antibody at 1:200 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM800070 requires heat-induced epitope retrieval with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The image shows strong cytoplasmic and membranous staining of the hepatocytes no staining in the bile duct. | |
Immunofluorescent staining of HepG2 cells using anti-ABAT mouse monoclonal antibody (UM800070, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). | |
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ABAT mouse monoclonal antibody (UM800070). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.(1:100) |
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