抗ATG13抗体 | Anti-ATG13 antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	IHC-P analysis of mouse heart tissue using GTX85183 ATG13 antibody. Working concentration : 20 μg/ml
	WB analysis of rat heart tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX85183 ATG13 antibody. Working concentration : 1 μg/ml
	IHC-P analysis of mouse heart tissue using GTX85183 ATG13 antibody. Working concentration : 5 μg/ml

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, ELISA
Reactivity	Human, Mouse, Rat

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1 μg/mL
IHC-P	5 μg/mL
ELISA	Assay dependent

Not tested in other applications.

Calculated MW	57 kDa. ( Note )
Positive Control	Rat Heart Tissue Lysate(GTX28912)

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PROPERTIES

Form	Liquid
Buffer	PBS with 0.02% sodium azide.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	ATG13 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ATG13.The immunogen is located within amino acids 450 - 500 of ATG13.
Purification	Purified by antigen-affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	autophagy related 13 , KIAA0652 , PARATARG8
Cellular Localization	Cytoplasm, cytosol
Background	Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG13 forms a complex with ULK1 and ULK2, the mammalian homologs of ATG1, and with FIP200. This complex is a target of TOR phosphorylation under normal conditions; inhibition of TOR by rapamycin or leucine deprivation leads to dephosphorylation of ATG13, ULK1 and ULK2, which then leads to autophagy. Knockdown of ATG13 inhibits autophagosome formation.
Database	・ Gene ID: 9776 ATG13 ・ UniProt: O75143 ATG13

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Stanton MJ et al. Cancer Res 2013; 73 (1) : 160-71 Autophagy control by the VEGF-C/NRP-2 axis in cancer and its implication for treatment resistance.	Application : WB Reactivity : Human

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