抗p53 [DO1]抗体 | Anti-p53 [DO1] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCE

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	Non-transfected (–) and transfected (+) HCT116 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
	Immunoprecipitation of p53 protein from 293T whole cell extracts using 5 μg of p53 antibody [DO1] (GTX70214). Western blot analysis was performed using p53 antibody [D01] (GTX70214). EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
	Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:10000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
	Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
	Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
	p53 antibody [DO1] detects p53 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
	p53 antibody [DO1] detects p53 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
	Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
	The WB analysis of p53 antibody [DO1] was published by Lee JH and colleagues in the journal PLoS One in 2014 . PMID: 24933654

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	DO1
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA
Reactivity	Human, Mouse

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:10000
ICC/IF	Assay dependent
IHC-P	1:1000
IHC-Fr	Assay dependent
FACS	Assay dependent
IP	1:100-1:500
ELISA	Assay dependent
Note : IHC-P Antigen retrieval can be done with EDTA at pH8.0 - Some customers have stated that the product works well without antigen retrieval.

Not tested in other applications.

Calculated MW	44 kDa. ( Note )
Positive Control	293T , A431 , HCT116 , 293T (0.5 μM doxorubicin treatment for 24 hr) , HCT116(30 μM cisplatin treatment for 24 hr)

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PROPERTIES

Form	Liquid
Buffer	PBS
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Recombinant human p53 expressed in E. coli.
Purification	Protein A purified From tissue culture supernatant
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Tumor Protein P53 , Bcc7 , Lfs1 , P53 , Trp53 , Tp53
Background	The p53 antibody, clone DO1, specifically recognizes an epitope at the N-terminal end of the human p53 protein between amino acids 20-25. p53 is a tumor suppressor gene that is mutated in a wide variety of sporadic and familial cancers. And p53, as a nuclear protein, mediates cell cycle arrest in response to DNA damage and therefore is important in the maintenance of genomic stability. Recent results indicate an enhanced phosphorylation of p53 by ATM in response to DNA damage on SER15, a mutation which reduces the ability of p53 to arrest cell growth.
Database	・ Gene ID: 7157 ・ UniProt: P04637

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REFERENCE

Application Reference	Application/Reactivity
Wang YY et al. Biochem Pharmacol 2020; 171 : 113713 Orabase-formulated gentian violet effectively improved oral potentially malignant disorder in vitro and in vivo.	Application : WB Reactivity : Human
Zhang Y et al. Oncol Lett 2019; 17 (1) : 127-134 Apoptosis of mouse myeloma cells induced by curcumin via the Notch3-p53 signaling axis.
Celegato M et al. Cancer Lett 2019; (Epub) A novel small-molecule inhibitor of the human papillomavirus E6-p53 interaction that reactivates p53 function and blocks cancer cells growth.	Application : WB Reactivity : Human
Chueh-Hsuan Lu et al. bioRxiv 2019; (Epub) Application of non-invasive low-intensity pulsed electric field with thermal cycling-hyperthermia for synergistically enhanced anticancer effect of chlorogenic acid on PANC-1 cells.	Application : WB Reactivity : Human
Li H et al. Cells 2019; 8 (9) PFN2a Suppresses C2C12 Myogenic Development by Inhibiting Proliferation and Promoting Apoptosis via the p53 Pathway.	Application : WB Reactivity : Human

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