抗Histone H4K8ac (acetyl Lys8) - ChIP grade抗体 | Anti-Histone H4K8ac (acetyl Lys8) - ChIP grade antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	ChIP was performed with U2OS nuclear extract and either 15 μl of H4K8ac antibody or 5 μg of control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to ALDOA promoter or MyoD.
	ELISA was performed using a serial dilution of Histone H4K8ac (acetyl Lys8) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:17,500.
	Dot blot analysis of peptides containing modified histone H4 and H3 and an unmodified histone H4 sequence uaing H4K8ac antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane for analysis.
	WB analysis of histone extracts (15 μg) from HeLa cells using H4K8ac antibody diluted 1:250 in TBS-Tween containing 5% skimmed milk. Lane 2 shows the result of the WB analysis with the antibody; lane 1 shows the same analysis after incubation of the antibody with 750 pmol blocking peptide for 1 hour at room temperature.

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製品情報

Host	Rabbit
Clonality	Polyclonal
Application	WB, Dot, ELISA, ChIP assay
Reactivity	Human

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APPLICATION

Application Note

ChIP (5 μl/ChIP), ELISA (1:500), Dot blotting (1:20,000), Western blotting (1:250)

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PROPERTIES

Form	Liquid
Buffer	Whole antiserum from rabbit containing 0.05% azide.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Purification	Whole antiserum from rabbit containing 0.05% azide.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Histone H4 acetyl Lys8 Antibody, H4FA Antibody, acetyl Histone H4 Antibody, histone cluster 1, H4a Antibody, HIST1H4A Antibody, acetyl Lys8 Histone H4 Antibody, Histone H4 acetyl Antibody, acetyl K8 Histone H4 Antibody, Histone H4 acetyl K8 Antibody

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