抗alpha Adaptin [AC1-M11]抗体 | Anti-alpha Adaptin [AC1-M11] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

目次に戻る

価格

目次に戻る

DATA IMAGES

	ICC/IF analysis of NRK cells using GTX22807 alpha Adaptin antibody [AC1-M11].
	IHC-P analysis of human heart tissue using GTX22807 alpha Adaptin antibody [AC1-M11]. Left : Primary antibodyRight : Negative control without primary antibodyAntigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutesDilution : 1:20
	ICC/IF analysis of SH-SY5Y cells using GTX22807 alpha Adaptin antibody [AC1-M11]. Panel e is a no primary antibody control.Green : Primary antibodyBlue : NucleiRed : ActinFixation : 4% paraformaldehydePermeabilization : 0.1% Triton X-100 for 10 minuteDilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature
	WB analysis of tissue and membrane enriched extract (30 ug) of Rat Brain (Lane 1), SH-SY5Y (Lane 2), U-87 MG (Lane 3), COLO 205 (Lane 4), PC-3 (Lane 5), MCF7 (Lane 6), NIH/3T3 (Lane 7) and A-431 (Lane 8) using GTX22807 alpha Adaptin antibody [AC1-M11]. Dilution : 2 μg/ml

目次に戻る

製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	AC1-M11
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, IP, IHC
Reactivity	Human, Mouse, Rat, Bovine, Chicken, Pig, Amphibians, Primate

目次に戻る

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	2 μg/mL
ICC/IF	2 μg/mL
IHC-P	1:20
IP	Assay dependent
IHC	Assay dependent

Not tested in other applications.

Specificity/Sensitivity	This antibody detects the products of both alpha-adaptin genes, alphaA and alphaC, as well as an alternatively spliced isoform of alphaA found in neurons.

目次に戻る

PROPERTIES

Form	Liquid
Buffer	PBS, 0.05% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Bovine
Immunogen	Purified bovine brain adaptor complexes.
Purification	Protein A purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

目次に戻る

TARGET

Synonyms	adaptor related protein complex 2 subunit alpha 1
Database	・ Gene ID: 784636 AP2A1

目次に戻る

REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Murakami N et al. PLoS One 2012; 7 (4) : e34845 Phosphorylation by Dyrk1A of clathrin coated vesicle-associated proteins: identification of the substrate proteins and the effects of phosphorylation.	Application : IP

目次に戻る