抗Fas抗体 | Anti-Fas antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	Immunohistochemical staining of human hepatoma tissue with Fas pAb.
	Western blot analysis of Fas pAb : Lane 1: Rat Brain Tissue Extract Lane 2: Mouse Brain Tissue Extract

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, FACS, IHC
Reactivity	Human, Mouse, Rat, Dog

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APPLICATION

Application Note

Recommended Starting Dilutions:
FACS: Use at an assay dependent dilution.
WB: Use at a dilution of 1:1000.
IHC: Use at a dilution of 1:50. Optimal dilution / concentration should be determined by the end user.

Calculated MW	37 kDa. ( Note )
Positive Control	HeLa Cell Lysate.

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PROPERTIES

Form	Liquid
Buffer	Preservative: 0.09% Sodium Azide; Constituents: PBS. pH 7.2
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Mouse
Immunogen	Synthetic peptide: ESLKLRRRVHETDKNC conjugated to KLH, corresponding to amino acids 29-44 of Mouse CD95 .
Purification	Immunogen affinity purified This antibody was purified on a peptide bound immunoaffinity column.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Fas (Tnf Receptor Superfamily Member 6) , Ai196731 , Apo1 , Apt1 , Cd95 , Tnfr6 , Tnfrsf6 , Lpr , Fas
Cellular Localization	Type I membrane protein (isoform 1); Secreted (isoforms 2 to 6).
Background	CD95, also known as FAS or APO-1, is a 36 kDa cell surface type I- membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE. CD95 is a member of the TNF receptor family, which includes TNFR-1, TNFR-2, CD27, CD30 and CD40. Binding of CD95 Ligand to CD95 or crosslinking of CD95 by anti-CD95 monoclonal antibodies leads to apoptosis of CD95 expressing cells. CD95 belongs to a subgroup of family members that have a death domain (DD) which contains 70 amino acids near the carboxyl-terminal region of the molecule. The binding of adaptor molecules to this DD is responsible for transmitting the death signal for apoptosis. Stimulation of CD95 results in aggregation of its DD, leading to the recruitment of FADD and caspase-8 that together with the receptor form the death-inducing signaling complex (DISC).
Database	・ Gene ID: 14102 ・ UniProt: P25446

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Peverelli E et al. Endocr Connect 2013; 2 (4) : 196-207 PKA regulatory subunit R2B is required for murine and human adipocyte differentiation.	Application : WB Reactivity : Human
Fellman CL et al. Vet Immunol Immunopathol 2011; 140 (43163) : 175-80 * a affects the in vitro expression of t cell activation-related molecules and cytokines in dogs.	Application : FACS Reactivity : Dog

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