抗Grp75 [30A5]抗体 | Anti-Grp75 [30A5] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	Western blot analysis of Grp75/Mortalin, mAb (30A5) (GTX13529): Lane 1: MW marker Lane 2: HeLa (heat shocked) Lane 3: L-929 (heat shocked) Lane 4: CHO -K1 (heat shocked) Lane 5: GPC-16 (heat shocked) Lane 6: PC-12 (heat shocked).
	Immunohistochemistry analysis of human breast cancer tissue immunohistochemically stained using Grp75 mAb (30A5).

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	30A5
Isotype	IgG1
Application	WB, ICC/IF, IP, IHC
Reactivity	Human, Mouse, Rat, Rabbit, Sheep, Bovine, Dog, Hamster, Guinea pig, Pig, Monkey, Xenopus laevis

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APPLICATION

Application Note

Immunohistochemistry (1:50) Western Blot (1:1000, colorimetric) Optimal conditions must be determined individually for each application.

Calculated MW	74 kDa. ( Note )
Positive Control	HeLa Cell Lysate (Heat Shocked)
Specificity/Sensitivity	This antibody detects an ~75 kDa protein, corresponding to the apparent molecular mass of Glucose Regulated Protein 75 (Grp75) on SDS-PAGE immunoblots.

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PROPERTIES

Form	Liquid
Buffer	Preservative: None; Constituents: 50% Glycerol, PBS, 0.1mM PMSF. pH 7.2
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Grp75 isolated from human Molt4 cells.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Heat Shock Protein Family A (Hsp70) Member 9 , Crp40 , Csa , Evpls , Grp-75 , Grp75 , Hel-S-124M , Hspa9B , Mot , Mot2 , Mthsp75 , Pbp74 , Saan , Sidba4 , Hspa9
Cellular Localization	Mitochondrial
Background	In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. One class of stress proteins, termed the Hsp70 family, is comprised of multiple members, all of which bind ATPin vitro, but which are localized within different intracellular compartments. These include: i) Hsc70 (or constitutive form) present within the cytosol/nucleus; ii) Hsp70 (inducible form) present within the cytosol/nucleus/nucleolus; iii) the constitutive glucose-regulated 78 kDa (or BiP) protein present within the lumen of the endoplasmic reticulum; and iv) the constitutive glucose regulated 75 kDa protein present within the mitochondrial matrix. Members of the Hsp70 family are thought to function as molecular chaperones, assisting in the folding of other proteins in various intracellular compartments. Grp75 is localized in the mitochondrial matrix, where, in concert with Hsp60, is thought to participate in the re-folding of proteins translocated into this organelle. Like its E. coli homolog DnaK, Grp75 possesses a cation-dependent ATPase activity thought to be central to its function as a chaperone.
Database	・ Gene ID: 3313 ・ UniProt: P38646

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