抗alpha Smooth Muscle Actin抗体 | Anti-alpha Smooth Muscle Actin antibody

掲載日情報:2020/04/13 現在Webページ番号:122055

GeneTex社の抗alpha Smooth Muscle Actin抗体 | Anti-alpha Smooth Muscle Actin antibodyです。

本製品は研究用です。研究用以外には使用できません。

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抗alpha Smooth Muscle Actin抗体(#GTX100034)のユーザーレビューはこちら。

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Application
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特長

  • 高品質の抗体です。
  • 幅広い研究分野に関連する抗体を取り揃えています。
  • 使用されたアプリケーションや動物種などの情報が充実しています。
  • 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

[在庫・価格 :2024年05月20日 12時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-ACTA2, Rabbit-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 91
説明文
レビューあり。KO/KDバリデーション済み抗体。
別名:actin alpha 2,smooth muscle,ACTSA
Genbank No: 59
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Human/Mouse/Rat/Zebrafish 適用 IC,IF,IHC,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 ゼブラフィッシュ(zebrafish)研究用製品特集
関連記事 GeneTex社における抗体の品質管理
Anti-ACTA2, Rabbit-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 91
説明文
レビューあり。KO/KDバリデーション済み抗体。
別名:actin alpha 2,smooth muscle,ACTSA
Genbank No: 59
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Human/Mouse/Rat/Zebrafish 適用 IC,IF,IHC,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 ゼブラフィッシュ(zebrafish)研究用製品特集
使いっきり抗体
関連記事 GeneTex社における抗体の品質管理

[在庫・価格 :2024年05月20日 12時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-ACTA2, Rabbit-Poly

文献数: 91

説明文 レビューあり。KO/KDバリデーション済み抗体。
別名:actin alpha 2,smooth muscle,ACTSA
Genbank No: 59
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Human/Mouse/Rat/Zebrafish 適用 IC,IF,IHC,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 ゼブラフィッシュ(zebrafish)研究用製品特集
関連記事 GeneTex社における抗体の品質管理

Anti-ACTA2, Rabbit-Poly

文献数: 91

説明文 レビューあり。KO/KDバリデーション済み抗体。
別名:actin alpha 2,smooth muscle,ACTSA
Genbank No: 59
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Human/Mouse/Rat/Zebrafish 適用 IC,IF,IHC,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 ゼブラフィッシュ(zebrafish)研究用製品特集
使いっきり抗体
関連記事 GeneTex社における抗体の品質管理



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DATA IMAGES


Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
alpha smooth muscle Actin antibody detects alpha smooth muscle Actin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha smooth muscle Actin antibody (GTX100034) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
alpha smooth muscle Actin antibody detects alpha smooth muscle Actin protein at cytoplasm on human liver carcinoma stroma by immunohistochemical analysis.
Sample: Paraffin-embedded liver carcinoma stroma.
alpha smooth muscle Actin antibody (GTX100034) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
alpha smooth muscle Actin antibody detects ACTA2 protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
10% SDS-PAGE
alpha smooth muscle Actin antibody (GTX100034) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse duodenum.alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat stomach.alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse duodenum.alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat cervix.alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat stomach.alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
The WB analysis of alpha Smooth Muscle Actin antibody was published by Chuang HM and colleagues in the journal Front Pharmacol in 2016 . PMID: 27199755
alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse prostate.Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat stomach.Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat cervix.Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse cervix.Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min


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製品情報

HostRabbit
ClonalityPolyclonal
IsotypeIgG
ApplicationWB, ICC/IF, IHC-P, IHC-Fr
ReactivityHuman, Mouse, Rat, Zebrafish


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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW 42 kDa. ( Note )
Positive Control HeLa , C8D30 , NIH-3T3 , C2C12 , Rat2 , *Mouse Heart
Predict Reactivity Rabbit, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)


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PROPERTIES

Form Liquid
Buffer 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration 0.39 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species Human
Immunogen Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human alpha Smooth Muscle Actin. The exact sequence is proprietary.
Purification Purified by antigen-affinity chromatography.
Conjugation Unconjugated
Note For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


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TARGET

Synonyms actin alpha 2, smooth muscle , ACTSA
Cellular Localization Cytoplasm , cytoskeleton
Background The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
Database ・ Gene ID: 59 ACTA2
・ UniProt: P62736 ACTA2


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REFERENCE

Application ReferenceApplication/Reactivity
Goto Y et al. J Oral Biosci 2020; (Epub) PLAG1 enhances the stemness profiles of acinar cells in normal human salivary glands in a cell type-specific manner.Application : IHC-Fr
Reactivity : Human
Lopez-Silva TL et al. Biomaterials 2020; 231 : 119667 Chemical functionality of multidomain peptide hydrogels governs early host immune response.Application : IHC-P
Reactivity : Rat
Sun Y et al. Med Sci Monit 2019; 25 : 6244-6254 SOX18 Affects Cell Viability, Migration, Invasiveness, and Apoptosis in Hepatocellular Carcinoma (HCC) Cells by Participating in Epithelial-to-Mesenchymal Transition (EMT) Progression and Adenosine Monophosphate Activated Protein Kinase (AMPK)/Mammalian Target of Rapamycin (mTOR).Application : WB
Reactivity : Human
Wang HC et al. Theranostics 2019; 9 (24) : 7168-7183 Pancreatic stellate cells activated by mutant KRAS-mediated PAI-1 upregulation foster pancreatic cancer progression via IL-8.Application : ICC/IF, IHC-P
Reactivity : Human, Mouse
Lin PH et al. J Pineal Res 2019; : e12620 Melatonin activates cell death programs for the suppression of uterine leiomyoma cell proliferation.Application : IHC-P
Reactivity : Rat


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