抗LGR5抗体(Anti-LGR5 Antibody clone 2A2)
掲載日情報：2019/10/29 現在Webページ番号：116145
OriGene社の抗LGR5 Mouse Monoclonal TrueMAB抗体です。
本製品は、TrueMAB抗体シリーズ(三次元構造を有する組換え体ヒトタンパク質の全長を抗原として作製したモノクローナル抗体)です。
※本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2024年05月19日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-LGR5, Mouse-Mono(2A2) TrueMAB |
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Anti-LGR5, Mouse-Mono(2A2), Carrier-free |
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[在庫・価格 ：2024年05月19日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-LGR5, Mouse-Mono(2A2) TrueMAB
文献数： 17
- 商品コード：TA503316
- メーカー：ORI
- 包装：100μl
- 価格：¥157,000
- 在庫：無(未発注)
- 納期：3～4週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
BSA/グリセロールフリー（キャリアフリー）タイプをご希望の場合は，商品コード#CF503316をお選び下さい．。 クローン：OTI2A2 Genbank No： 8549 Gene Accession No： NM_003667 |
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| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Human | 免疫動物 | Mouse |
| 交差性 | Human/Mouse/Rat | 適用 | FCM,IF,IHC,IP,Western Blot |
| 標識 | Unlabeled | 性状 | Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Monoclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 |
Anti-LGR5 Antibody（UltraMAB / TrueMABシリーズ） TrueMAB抗体 |
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| 関連記事 | |||
Anti-LGR5, Mouse-Mono(2A2), Carrier-free
文献数： 0
- 商品コード：CF503316
- メーカー：ORI
- 包装：100μg
- 価格：¥197,000
- 在庫：無(未発注)
- 納期：3～4週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
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特長
OriGene社は50,000点以上の1次抗体を提供しており、最先端の抗原精製技術により、高品質な抗体をお届けしています。一部抗体製品にはウェスタンブロット用のポジティブコントロールが同梱されます。
OriGene社抗体製品の中でも特にTrueMAB 抗体シリーズは、三次元構造を有する組換え体ヒトタンパク質の全長を抗原として作製したモノクローナル抗体です。そのため、立体構造を持たない短鎖ペプチドを抗原として作製した抗体では検出が困難なエピトープも認識でき、ネイティブなタンパク質への特異性が向上しています。
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使用例
 | Figure from citation: Positive correlation between LSD1, c-Myc, ß-catenin and LGR5 expression in human colorectal tumor tissues. Expression levels of LSD1, LGR5, ß-catenin and c-Myc in consecutive sections from normal colon, adenoma and CRC tumor tissues. Dilution: 1:100 View Citation |
 | Figure from citation: Immunohistochemical analysis of Lgr5 expression in human normal colon biopsies; Lgr5+ cells are localized at the crypt base, where bona fide stem-like cells home; magnification is indicated in the boxes. Dilution: 1:200 View Citation |
 | Figure from citation: Immunohistochemical staining of Lgr5 in normal gastric tissues. The normal gastric tissue, 40×magnified (A) and 400× magnified (B). Yellowspots represent the Lgr5 positive cells that are localized only at base of the glands in normal gastric mucosa. The arrows in the pictures show the immunoreactive cells stained in yellow color. View Citation |
 | Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503316) |
 | Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503316) |
 | Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503316) |
 | Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503316) |
 | Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503316) |
 | Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503316) |
 | HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR5 (RC212825, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5. Positive lysates LY401213 (100ug) and LC401213 (20ug) can be purchased separately from OriGene. |
 | Immunoprecipitation(IP) of LGR5 by using TrueMab monoclonal anti-LGR5 antibodies (Negative control: IP without adding anti-LGR5 antibody.). For each experiment, 500ul of DDK tagged LGR5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-LGR5 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody. |
 | Anti-LGR5 mouse monoclonal antibody (TA503316) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGR5(RC212825). |
 | Figure from citation: Immunocytochemistry of epithelium stem cell phenotype. Isolated dental epithelium following 5-day culture, overwhelmingly expressed Lgr5. Dilution: 1:100 View Citation |
 | Immunofluorescent staining of HT29 cells using anti-LGR5 mouse monoclonal antibody (TA503316, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). |
 | HEK293T cells transfected with either RC212825 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LGR5 antibody(TA503316), and then analyzed by flow cytometry. |
 | Figure from citation: Cytofluorimetric analysis of Lgr5 expression by PKH high cells over 4 weeks of culture. Data are expressed as percent mean values (6 SD) of 5 consecutive experiments. Dilution: 1:400 View Citation |
 | Figure from citation: Flow cytometry of the SB cells. The G4 region was further divided into P1 and P2. Lgr5, a stem cell marker, was expressed by 32% of the P2 population. Black: staining with Lgr5; red: staining with the isotype control. View Citation |
 | Figure from citation: [Nature] Cells expressing the stem cell marker Lgr5 was detected by immunostaining of hCECs in 2D culture. View Citation Pubmed id: 27398792 Memo: Human; IF |
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製品情報
| Clone Name | OTI2A2 |
|---|---|
| Applications | WB, IHC, IF, IP, FC |
| Recommend Dilution | WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100, IP 2ug/500ul |
| Reactivity | Human, Mouse |
| Host | Mouse |
| Clonality | Monoclonal |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 250-550 of human LGR5 (NP_003658) produced in HEK293T Cell. |
| Isotype | IgG1 |
| Formulation | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
| Concentration | 1 mg/ml |
| Purification | Purified from mouse ascites fluids by affinity chromatography |
| Predicted Protein Size | 99.8 kDa |
| Gene Name | leucine-rich repeat containing G protein-coupled receptor 5 |
| Synonyms | FEX|GPR49|GPR67|GRP49|HG38 |
| Database Link |
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文献
Resveratrol reduces senescence-associated secretory phenotype by SIRT1/NF-κB pathway in gut of the annual fish Nothobranchius guentheri ,Liu, S;Zheng, Z;Ji, S;Liu, T;Hou, Y;Li, S;Li, G;, Fish Shellfish Immunol. 2018 ,PubMed ID 29908321 [LGR5]NANOGP8 is the key regulator of stemness, EMT, Wnt pathway, chemoresistance, and other malignant phenotypes in gastric cancer cells ,Ma, X;Wang, B;Wang, X;Luo, Y;Fan, W;, PLoS ONE 2018 ,PubMed ID 29689047 [LGR5]
Liver damage, proliferation, and progenitor cell markers in experimental necrotizing enterocolitis ,Miyake, H;Li, B;Lee, C;Koike, Y;Chen, Y;Seo, S;Pierro, A;, J. Pediatr. Surg. 2018 ,PubMed ID 29502797 [LGR5]
Identification of PMN-released Mutagenic Factors in a Co-culture Model for Colitis-associated Cancer ,Granofszky, N;Lang, M;Khare, V;Schmid, G;Scharl, T;Ferk, F;Jimenez, K;Knasmüller, S;Campregher, C;Gasche, C;, Carcinogenesis 2017 ,PubMed ID 29106440 [LGR5]
Immuno-proteomic discovery of tumor tissue autoantigens identifies olfactomedin 4, CD11b, and integrin alpha-2 as markers of colorectal cancer with liver metastases ,Yang, Q;Bavi, P;Wang, JY;Roehrl, MH;, J Proteomics 2017 ,PubMed ID 28669815 [LGR5]
LGR5 Is a Gastric Cancer Stem Cell Marker Associated with Stemness and the EMT Signature Genes NANOG, NANOGP8, PRRX1, TWIST1, and BMI1 ,Wang, B;Chen, Q;Cao, Y;Ma, X;Yin, C;Jia, Y;Zang, A;Fan, W;, PLoS ONE 2016 ,PubMed ID 28033430 [LGR5]
Heparan sulfate hexasaccharide selectively inhibits cancer stem cells self-renewal by activating p38 MAP kinase ,Patel, NJ;Sharon, C;Baranwal, S;Boothello, RS;Desai, UR;Patel, BB;, Oncotarget 2016 ,PubMed ID 27705927 [LGR5]
A recellularized human colon model identifies cancer driver genes ,Chen, HJ;Wei, Z;Sun, J;Bhattacharya, A;Savage, DJ;Serda, R;Mackeyev, Y;Curley, SA;Bu, P;Wang, L;Chen, S;Cohen-Gould, L;Huang, E;Shen, X;Lipkin, SM;Copeland, NG;Jenkins, NA;Shuler, ML;, Nat. Biotechnol. 2016 ,PubMed ID 27398792 [LGR5]
Full-length LGR5-positive cells have chemoresistant characteristics in colorectal cancer ,Osawa, H;Takahashi, H;Nishimura, J;Ohta, K;Haraguchi, N;Hata, T;Yamamoto, H;Mizushima, T;Takemasa, I;Doki, Y;Mori, M;, Br. J. Cancer May 2016 ,PubMed ID 27140312 [LGR5]
LGR5 activates non-canonical Wnt-signaling and inhibits aldosterone production in the human adrenal ,Shaikh, LH;Zhou, J;Teo, AE;Garg, S;Neogi, SG;Figg, N;Yeo, GS;Yu, H;Maguire, JJ;Zhao, W;Bennett, MR;Azizan, EA;Davenport, AP;McKenzie, G;Brown, MJ;, J. Clin. Endocrinol. Metab. Apr 2015 ,PubMed ID 25915569 [LGR5]
CBB1003, a lysine-specific demethylase 1 inhibitor, suppresses colorectal cancer cells growth through down-regulation of leucine-rich repeat-containing G-protein-coupled receptor 5 expression ,Hsu, HC;Liu, YS;Tseng, KC;Yang, TS;Yeh, CY;You, JF;Hung, HY;Chen, SJ;Chen, HC;, J. Cancer Res. Clin. Oncol. July 2014 ,PubMed ID 25060070 [LGR5]
[Human; IHC]LGR5 regulates survival through mitochondria-mediated apoptosis and by targeting the Wnt/ß-catenin signaling pathway in colorectal cancer cells ,Hsu, HC;Liu, YS;Tseng, KC;Tan, BC;Chen, SJ;Chen, HC;, Cell. Signal. July 2014 ,PubMed ID 25025569 [LGR5]
[Human; WB]Identification of a distinct small cell population from human bone marrow reveals its multipotency in vivo and in vitro ,Wang, J;Guo, X;Lui, M;Chu, PJ;Yoo, J;Chang, M;Yen, Y;, PLoS ONE, Jan 2014. ,PubMed ID 24465489 [LGR5]
[Human; FC]Postnatal epithelium and mesenchyme stem/progenitor cells in bioengineered amelogenesis and dentinogenesis ,Jiang, N;Zhou, J;Chen, M;Schiff, MD;Lee, CH;Kong, K;Embree, MC;Zhou, Y;Mao, JJ;, Biomaterials Dec 2013. ,PubMed ID 24345734 [Lgr5]
[Rat; IF]Lgr5 expression as stem cell marker in human gastric gland and its relatedness with other putative cancer stem cell markers ,Chen Wu, Yuanyuan Xie, Feng Gao, Yanan Wang, Yawei Guo, Huanna Tian, Yanshuang Li, Wufang Fan, Gene. 2013 Aug 1;525(1):18-25 ,PubMed ID 23664892 [LGR5]
PKH26 staining defines distinct subsets of normal human colon epithelial cells at different maturation stages ,Pastò A, Marchesi M, Diamantini A, Frasson C, Curtarello M, Lago C, Pilotto G, Parenti AR, Esposito G, Agostini M, Nitti D, Amadori A, PLoS One. 2012;7(8):e43379 ,PubMed ID 22927961 [LGR5]
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