抗ARNT, Mouse-Mono(OTI1A1) TrueMAB抗体 | Anti-ARNT, Mouse-Mono(OTI1A1) TrueMAB Antibody
掲載日情報:2019/10/29 現在Webページ番号:114277
OriGene社の抗ARNT, Mouse-Mono(OTI1A1) TrueMAB抗体です。
本製品は、TrueMAB抗体シリーズ(三次元構造を有する組換え体ヒトタンパク質の全長を抗原として作製したモノクローナル抗体)です。
※本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 :2024年06月12日 20時15分現在]
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[在庫・価格 :2024年06月12日 20時15分現在]
Anti-ARNT, Mouse-Mono(OTI1A1) TrueMAB
文献数: 0
- 商品コード:TA501147S
- メーカー:ORI
- 包装:30μl
- 価格:¥68,000
- 在庫:無(未発注)
- 納期:3~4週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
Anti-ARNT, Mouse-Mono(1A1) TrueMAB
文献数: 2
- 商品コード:TA501147
- メーカー:ORI
- 包装:100μl
- 価格:¥150,000
- 在庫:無(未発注)
- 納期:3~4週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
BSA/グリセロールフリー(キャリアフリー)タイプをご希望の場合は,商品コード#CF501147をお選び下さい.。 クローン:OTI1A1 Genbank No: 405 Gene Accession No: NM_001668 |
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保存条件 | 法規備考 | ||
抗原種 | Human | 免疫動物 | Mouse |
交差性 | Human/Mouse/Rat | 適用 | Western Blot,IHC,IF,FCM |
標識 | Unlabeled | 性状 | Affinity Purified |
吸収処理 | クラス | IgG | |
クロナリティ | Monoclonal | フォーマット | |
掲載カタログ |
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製品記事 |
TrueMAB抗体 |
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関連記事 |
Anti-ARNT, Mouse-Mono(1A1), Carrier-free
文献数: 0
- 商品コード:CF501147
- メーカー:ORI
- 包装:100μg
- 価格:¥201,000
- 在庫:無(未発注)
- 納期:3~4週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
追加しました。
特長
OriGene社は50,000点以上の1次抗体を提供しており、最先端の抗原精製技術により、高品質な抗体をお届けしています。一部抗体製品にはウェスタンブロット用のポジティブコントロールが同梱されます。
OriGene社抗体製品の中でも特にTrueMAB 抗体シリーズは、三次元構造を有する組換え体ヒトタンパク質の全長を抗原として作製したモノクローナル抗体です。そのため、立体構造を持たない短鎖ペプチドを抗原として作製した抗体では検出が困難なエピトープも認識でき、ネイティブなタンパク質への特異性が向上しています。
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使用例
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARNT (RC216724, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARNT. Positive lysates LY400636 (100ug) and LC400636 (20ug) can be purchased separately from OriGene. | |
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-ARNT monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen). | |
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501147) | |
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501147) | |
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501147) | |
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501147) | |
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501147) | |
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501147) | |
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501147) | |
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501147) | |
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501147) | |
Anti-ARNT mouse monoclonal antibody (TA501147) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARNT(RC216724). | |
Figure from citation: Immunofluorescence of ARNT protein level by using anti-ARNT antibody in mouse neuronal cells. View Citation | |
HEK293T cells transfected with either RC216724 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ARNT antibody(TA501147), and then analyzed by flow cytometry. | |
Flow cytometric Analysis of Jurkat cells, using anti-ARNT antibody(TA501147),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue). |
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製品情報
Clone Name | OTI1A1 |
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Applications | WB, IHC, IF, FC |
Recommend Dilution | WB 1:2000, IHC 1:50, IF 1:100, FLOW 1:100 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Full length human recombinant protein of human ARNT (NP_001659) produced in HEK293T cell. |
Isotype | IgG1 |
Formulation | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
Concentration | 1.00mg/ml |
Purification | Purified from cell culture supernatant by affinity chromatography |
Predicted Protein Size | 86.5 kDa |
Gene Name | aryl hydrocarbon receptor nuclear translocator |
Background | The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1). A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Three alternatively spliced variants encoding different isoforms have been described for this gene. |
Synonyms | HIF-1-beta|HIF-1beta|HIF1-beta|HIF1B|HIF1BETA|TANGO|bHLHe2 |
Database Link |
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文献
Reciprocal regulation of the basic helix–loop–helix/Per–Arnt–Sim partner proteins, Arnt and Arnt2, during neuronal differentiation ,Nan Hao, Veronica L. D. Bhakti, Daniel J. Peet, and Murray L. Whitelaw, Nucleic Acids Res., Jun 2013; 41: 5626 - 5638. ,PubMed ID 23599003 [ARNT][Mouse; IF]
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