Human CXCL8/IL-8 QuantiGlo ELISA Kit
掲載日情報：2016/08/18 現在Webページ番号：111380
R&D Systems製のHuman CXCL8/IL-8を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年06月05日 00時00分現在]
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IL-8, Human, ELISA Kit, QuantiGlo, 2nd Generation (96well) <CXCL8, ELISA Kit> |
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[在庫・価格 ：2024年06月05日 00時00分現在]
IL-8, Human, ELISA Kit, QuantiGlo, 2nd Generation (96well) <CXCL8, ELISA Kit>
文献数： 12
- 商品コード：Q8000B
- メーカー：RSD
- 包装：1kit
- 価格：¥135,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：1.60 - 5000 pg/mL，感度：0.97 pg/mL，測定試料：Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Citrate Plasma (50 uL)，種交差性：Human，検出方法：化学発光，アッセイ法：サンドイッチELISA，アッセイ数：96 well，別売品コントロール：#QC194，測定時間：5 hours，測定因子：CXCL8/IL-8 別名：3-10C Genbank No： 3576 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
QuantiGlo Chemiluminescent Sandwich ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Citrate Plasma (50 µL)
- Sensitivity
- 0.97 pg/mL
- Assay Range
- 1.6 - 5,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Citrate Plasma, EDTA Plasma)
- Specificity
- Natural and recombinant human IL-8
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The QuantiGlo Human IL-8 Chemiluminescent Immunoassay is a 5 hour solid phase ELISA designed to measure human IL-8 levels in cell culture supernates, serum, and plasma. It contains the 72 amino acid variant of human IL-8 derived from E. coli as well as antibodies raised against this recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-8. Results obtained using natural human IL-8 showed linear curves that were parallel to the standard curves obtained using the QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-8.
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Precision
Intra-Assay Precision (Precision within an assay)
Four samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Four samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||||
| Sample | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 |
| n | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 3.66 | 22.5 | 228 | 2,263 | 4.19 | 24.9 | 243 | 2,393 |
| Standard Deviation | 0.241 | 1.02 | 8.98 | 146 | 0.481 | 1.57 | 14.7 | 216 |
| CV% | 6.6 | 4.5 | 3.9 | 6.5 | 11.5 | 6.3 | 6 | 9 |
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Recovery
The recovery of IL-8 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 103 | 98-107 |
| Serum (n=4) | 103 | 93-107 |
| EDTA Plasma (n=4) | 105 | 95-113 |
| Heparin Plasma (n=4) | 107 | 102-113 |
| Citrate Plasma (n=4) | 101 | 97-107 |
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Linearity
To assess the linearity of the assay, samples spiked with high concentrations of IL-8 in various matrices were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- CXCL8/IL-8
- Aliases
- GCP1; IL8; LAI; MDNCF; NAP1; NCF; TCF; TSG1
- Entrez Gene IDs
- 3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
- Background
- CXCL8/IL-8
- CXCL8 was originally discovered and purified as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocytederived neutrophil chemotactic factor (MDNCF), T lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce CXCL8 in response to a wide variety of proinflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. CXCL8 is a member of the alpha (CXC) subfamily of chemokines, which also includes platelet factor-4, GRO, and IP10.CXCL8 is a potent chemoattractant for neutrophils. In addition, CXCL8 also has a wide range of other proinflammatory effects. CXCL8 causes degranulation of neutrophil specific granules and azurophilic granules. CXCL8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and subendothelial matrix proteins. Besides neutrophils, CXCL8 is also chemotactic for basophils, T cells and eosinophils. CXCL8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. CXCL8 was also reported to be angiogenic both in vivo and in vitro.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Activation of nuclear factor-kappa B signalling promotes cellular senescence.
Alvarez-Fernandez M, Caballero OL, Caetano C, Hummerich H, Jat PS, Mansfield L, Medema RH, Rovillain E
Oncogene 2011 30:2356-66
Species: Human
Sample Type: Cell Culture Supernates
Procalcitonin as an early marker of bacterial infection in neutropenic febrile children with acute lymphoblastic leukemia.
Athanassiadou F, Catriu D, Hatzistilianou M, Rekliti A
Inflamm. Res. 2010 59:339-47
Species: Human
Sample Type: Serum
Phase I clinical and pharmacodynamic evaluation of oral CI-1033 in patients with refractory cancer.
Christensen J, Donato NJ, Eiseman I, Huang X, Lenehan PF, Nemunaitis J, Olson SC, Shin DM, Shin HJ, Zinner RG
Clin. Cancer Res. 2007 13:3006-14
Species: Human
Sample Type: Plasma
Presence of cytokeratins in exhaled breath condensate of mechanical ventilated patients.
Brettschneider S, Dihazi H, Engelmann L, Eschrich K, Gessner C, Hammerschmidt S, Keller T, Kuhn H, Sack U, Wirtz H
Respir Med 2007 102:299-306
Species: Human
Sample Type: EBC
Recombinant human antithrombin inhibits thrombin formation and interleukin 6 release in human endotoxemia.
Firbas C, Jilma B, Leitner JM, Mayr FB, Reiter RA, Steinlechner B
Clin. Pharmacol. Ther. 2006 79:23-34
Species: Human
Sample Type: Plasma
Trichomonas vaginalis lipophosphoglycan triggers a selective upregulation of cytokines by human female reproductive tract epithelial cells.
Costello CE, Fichorova RN, Gilbert RO, Hayes GR, Lucas JJ, Singh BN, Trifonova RT
Infect. Immun. 2006 74:5773-9
Species: Human
Sample Type: Cell Culture Supernates
Endurance training reduces circulating inflammatory markers in persons at risk of coronary events: impact on plaque stabilization?
Heeb-Elze E, Huber K, Leitner-Heinschink A, Niessner A, Niessner C, Penka M, Richter B, Steiner S, Strasser B, Wojta J, Ziegler S, Zorn G
Atherosclerosis 2005 186:160-5
Species: Human
Sample Type: Plasma
Passive transfer of scrub typhus plasma to patients with AIDS: a descriptive clinical study.
Burnouf T, de Souza M, Jongsakul K, Kantipong P, Watt G
QJM 2001 94:599-607
Species: Human
Sample Type: Plasma
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