Human IL-2 QuantiGlo ELISA Kit
掲載日情報：2016/08/18 現在Webページ番号：111378
R&D Systems製のHuman IL-2を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月29日 18時55分現在]
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IL-2, Human, ELISA Kit, QuantiGlo, 2nd Generation (96well) |
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[在庫・価格 ：2024年05月29日 18時55分現在]
IL-2, Human, ELISA Kit, QuantiGlo, 2nd Generation (96well)
文献数： 0
- 商品コード：Q2000B
- メーカー：RSD
- 包装：1kit
- 価格：¥135,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：1.70 - 1250 pg/mL，感度：0.25 pg/mL，測定試料：Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)，種交差性：Human，検出方法：化学発光，アッセイ法：サンドイッチELISA，アッセイ数：96 well，別売品コントロール：#QC191，測定時間：5.5 hours，測定因子：IL-2 別名：aldesleukin Genbank No： 3558 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 |
QuantiGlo Chemiluminescent Sandwich ELISA Kit 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） Helper T Cells Marker Antibodies |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 5.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
- Sensitivity
- 0.25 pg/mL
- Assay Range
- 1.7 - 1,250 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant human IL-2
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The QuantiGlo Human IL-2 Chemiluminescent Immunoassay is a 5.5 hour solid phase ELISA designed to measure IL-2 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-2 and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human IL-2. Results obtained using natural human IL-2 showed linear curves that were parallel to the standard curves obtained using the recombinant QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-2.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 5.8 | 175 | 884 | 5.9 | 165 | 795 |
| Standard Deviation | 0.19 | 5.6 | 23 | 0.46 | 11 | 59 |
| CV% | 3.3 | 3.2 | 2.6 | 7.8 | 6.7 | 7.4 |
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Recovery
The recovery of IL-2 spiked to three different levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 102 | 99-105 |
| Serum (n=4) | 98 | 96-101 |
| EDTA Plasma (n=4) | 97 | 95-100 |
| Heparin Plasma (n=4) | 97 | 94-104 |
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Linearity
To assess the linearity of the assay, four samples containing or spiked with high concentrations of IL-2 in various matrices were diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- IL-2
- Aliases
- undefined
- Entrez Gene IDs
- 3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
- Background
- IL-2
- Interleukin 2 (IL-2) was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens. Since then, it has also been shown to stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages and oligodendrocytes. At the amino acid sequence level, there is approximately 60% - 90% similarity between species. Mature human IL-2 shows 65%, 67%, 72%, 78%, and 64% aa identity to mouse, rat, pig, cat, and cow IL-2, respectively.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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