Mouse MFG-E8 Quantikine ELISA Kit | mMFG-E8 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111297
R&D Systems製のMouse MFG-E8を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月20日 18時35分現在]
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[在庫・価格 ：2024年05月20日 18時35分現在]
Mouse MFG-E8 Quantikine ELISA Kit
文献数： 5
- 商品コード：MFGE80
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：0.13 - 8 ng/mL，感度：0.02 ng/mL，測定試料：Cell Culture Supernates (25 uL), Serum (10 uL), Heparin Plasma (10 uL)，種交差性：Mouse，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：MFG-E8 別名：BA46 Genbank No： 4240 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 |
Recombinant Human BMP-2/BMP-6 Heterodimer ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (25 µL), Serum (10 µL), Heparin Plasma (10 µL)
- Sensitivity
- 0.02 ng/mL
- Assay Range
- 0.125 - 8 ng/mL
- Specificity
- Natural and recombinant mouse MFG-E8
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse MFG-E8 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse MFG-E8 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse MFG-E8 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse MFG-E8 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse MFG-E8.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 0.506 | 0.936 | 3.89 | 0.509 | 0.91 | 3.78 |
| Standard Deviation | 0.02 | 0.042 | 0.116 | 0.047 | 0.071 | 0.322 |
| CV% | 4 | 4.5 | 3 | 9.2 | 7.8 | 8.5 |
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Recovery
The recovery of MFG-E8 spiked into various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Supernates (n=4) | 99 | 90-104 |
| Serum (n=4) | 101 | 97-104 |
| Heparin Plasma (n=4) | 94 | 90-98 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse MFG-E8 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- MFG-E8
- Aliases
- BA46; Breast epithelial antigen BA46; Lactahedrin; Medin; SED1
- Entrez Gene IDs
- 4240 (Human); 17304 (Mouse)
- Background
- MFG-E8
- MFG-E8, originally identified as a product of lactating mammary glands, is also secreted by activated macrophages and immature dendritic cells. MFG-E8 functions as an opsonin by binding phosphatidylserine on apoptotic cells and enhancing their phagocytosis.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
The neuroprotective effects of milk fat globule-EGF factor 8 against oligomeric amyloid beta toxicity.
J Neuroinflammation 2012 9:148
Species: Mouse
Sample Type: Cell Culture Supernates
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