Mouse/Rat FGF basic Quantikine ELISA Kit | m/rFGF basic Qkit
掲載日情報：2016/08/18 現在Webページ番号：111296
R&D Systems製のMouse/Rat FGF basicを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年06月08日 00時01分現在]
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Mouse/Rat FGF basic/FGF2 Quantikine ELISA Kit |
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[在庫・価格 ：2024年06月08日 00時01分現在]
Mouse/Rat FGF basic/FGF2 Quantikine ELISA Kit
文献数： 20
- 商品コード：MFB00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
感度：3.68 pg/mL，測定範囲：15.60 - 1000 pg/mL，測定試料：Cell Culture Supernates (50 uL), Tissue Lysates (10 uL), Serum (50 uL), EDTA Plasma (50 uL)，種交差性：Mouse/Rat，測定波長：450 nm，検出方法：呈色，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：FGF basic/FGF2/bFGF 別名：Basic fibroblast growth factor，bFGF Genbank No： 2247 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
Quantikine Mouse/Rat FGF Basic ELISA Kit Fluorochrome-Conjugated Human Arginase 1 Monoclonal Antibodies ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Tissue Lysates (10 µL), Serum (50 µL), EDTA Plasma (50 µL)
- Sensitivity
- 3.68 pg/mL
- Assay Range
- 15.6 - 1,000 pg/mL (Serum, Cell Culture Supernates, EDTA Plasma, Tissue Lysates)
- Specificity
- Natural and recombinant FGF basic.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse/Rat FGF basic Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse or rat FGF basic in cell culture supernates, tissue lysates, serum, and plasma. It contains E. coli-expressed recombinant rat FGF basic and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural FGF basic showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural FGF basic.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 49.5 | 122 | 394 | 45 | 104 | 382 |
| Standard Deviation | 1.17 | 2.7 | 12 | 3.8 | 5.03 | 20.5 |
| CV% | 2.4 | 2.2 | 3.1 | 8.4 | 4.8 | 5.4 |
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Recovery
The recovery of FGF basic spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 95 | 84-110 |
| Cell Culture Samples (n=4) | 101 | 91-110 |
| Tissue Lysates (n=4) | 97 | 80-106 |
| EDTA Plasma (n=4) | 97 | 84-110 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of FGF basic were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- FGF basic
- Aliases
- FGF2; FGF-2; FGF2AS; GFG1; HBGH-2; NUDT6; Prostatropin
- Entrez Gene IDs
- 2247 (Human); 14173 (Mouse); 281161 (Bovine)
- Background
- FGF basic
- View FGF Superfamily SchematicThe Fibroblast Growth Factors (FGFs) constitute a large family of proteins involved in many aspects of development including cell proliferation, growth, and differentiation. They act on several cell types to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects, and tissue repair. FGF family activities are mediated by receptor tyrosine kinases and are facilitated by heparan sulfate. Family members have been implicated in several disorders of bone growth, as well as in tumor formation and progression.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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