Human CA125/MUC16 Quantikine ELISA Kit | hCA125 QKit
掲載日情報：2016/08/18 現在Webページ番号：111037
R&D Systems製のHuman CA125/MUC16を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年06月13日 20時55分現在]
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Human CA125/MUC16 Quantikine ELISA Kit |
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[在庫・価格 ：2024年06月13日 20時55分現在]
Human CA125/MUC16 Quantikine ELISA Kit
文献数： 4
- 商品コード：DCA125
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
種交差性：Human，感度：0.099 U/mL，測定範囲：0.50 - 32 U/mL，測定試料：Cell Culture Supernates (100 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (10 uL), Urine (100 uL), Human Milk (10 uL)，測定波長：450 nm，検出方法：呈色，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC56，測定時間：4.5 hours，測定因子：CA125/MUC16 別名：CA-125 Genbank No： 94025 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 |
Quantikine Human CA125 ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Saliva (10 µL), Urine (100 µL), Human Milk (10 µL)
- Sensitivity
- 0.099 U/mL
- Assay Range
- 0.5 - 32 U/mL (Serum, Heparin Plasma, Saliva, Cell Culture Supernates, Human Milk, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human CA125
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human CA125/MUC16 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CA125 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains CHO cell-expressed recombinant human CA125 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CA125 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CA125.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 4.26 | 10 | 19 | 4.22 | 9.74 | 18.7 |
| Standard Deviation | 0.066 | 0.101 | 0.266 | 0.313 | 0.527 | 0.666 |
| CV% | 1.5 | 1 | 1.4 | 7.4 | 5.4 | 3.6 |
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Recovery
The recovery of CA125 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 99 | 95-102 |
| Serum (n=4) | 97 | 90-101 |
| EDTA Plasma (n=4) | 97 | 93-101 |
| Heparin Plasma (n=4) | 97 | 92-100 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of CA125 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- CA125/MUC16
- Aliases
- MUC16
- Entrez Gene IDs
- 94025 (Human)
- Background
- CA125
- The CA125 antigen, also known as MUC16, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. Expression of isoforms, proteolytic cleavage, and heavy N- and O- linked glycosylation produce forms of human CA125 that can vary from 200-5000 kDa in size. It inhibits NK cell activity, which is thought to contribute to immune evasion in peritoneal cancer and pregnancy.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
False biomarker discovery due to reactivity of a commercial ELISA for CUZD1 with cancer antigen CA125.
Blasutig , Ivan M, Brand , Randall, Brinc , Davor, Chrystoja , Caitlin , Diamandis , Elefther, Dimitromanolakis , Apostolo, Farkona , Sofia, Kulasingam , Vathany, Leung , Felix, Prassas , Ioannis
Clin Chem 2014 60:381-8
Species: Human
Sample Type: Serum
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