Quinacrine Inhibitor [NBP2-29385] - Evaluation of inhibitory activity of quinacrine. TLR9/NF-kB/SEAPorter? HEK 293 and TLR2/NF-kB/SEAPorter? HEK 293 cells were plated in 96-well plates at 5x10^4 cells/well for 16 h. Cells were preincubated with different concentrations of quinacrine (0.05, 0.1, 0.5, 1, 5 and 10 uM) for 30 min. Cell were then stimulated with 10 ug/ml CpG [A] or 20 ng/ml MALP-2 [B] for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter? Assay Kit