Ligand Activation: Human TLR4/MD-2/CD14 SEAP - (SEAPorter?) Stable Reporter Cell Line [NBP2-26503] - TLR4/MD-2/CD14 reporter cell line was plated in 96-well plates at 1 x 10^5 cells/well. After 16 h, cells were stimulated with various amount of LPS for 24 h. SEAP was analyzed using the SEAPorter Assay Kit. A. LPS-mediated TLR4 activation, as measured by SEAP activity, was increased in a dose dependent manner. B. The values from (A) were used to determine the EC50 of LPS. The EC50, or the half maximal activity concentration, represents the concentration of LPS that was required for 50% activation of TLR4 as measured by SEAP activity. Data Summary: The cell line was activated by LPS in a dose-response manner, of which EC50 was measured as 8.98 ng/ml.