Ligand Activation: Human IL17 Luciferase - (LUCPorter?) Stable Reporter Cell Line [NBP2-26283] - Plated in 24-well plates at 0.2 x 10^6 cells/well. After 16 h, cells were transiently transfected with RORgt [del.5-8]* plasmid (IMP-124) at 0.4 ug/well for 24 h. Luciferase activity was then analyzed using the luciferase reporter assay reagent. RU = response unit. A, Total luciferase RU data. B, Relative luciferase RU data. * RORgt [del5-8] = An RORgt isoform which lacks the hinge-encoding exons 5-8. Note: The luciferase reporter assay reagent is designed to be directly added to the cell culture plate without any additional steps when a luminescence assay-compatible white plate is used. After 5-10 min, the white plate can directly be analyzed by reading in a plate luminometer.