Immunoprecipitation: TFEB Antibody [NBP2-12758] - Samples: Whole cell lysate from HeLa (50 ug for WB; 1 mg for IP, 20% of IP loaded). For WB, samples were collected from HeLa cells expressing one of two shRNA for TFEB (shA or shB) or expressing a scrambled shRNA (shS). For IP, whole cell lysate was prepared from HeLa cells expressing the scrambled shRNA. Antibodies: Affinity purified rabbit anti-TFEB antibody NBP2-12758 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. TFEB was also immunoprecipitated by rabbit anti-TFEB antibody NBP2-12759, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).