Dot Blot: Porcine IgG, H/L Chains Antibody [Fluorescein] [NBP1-73089] Dot Blot showing the detection of Swine IgG. A three-fold serial dilution of Swine IgG starting at 200ng was spotted onto 0.45 um nitrocellulose. After blocking in Blocking Buffer for Fluorescent Western Blotting 1 Hour at 20C, Anti-Swine IgG (H&L) (GOAT) Antibody Fluorescien Conjugated secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting and imaged using Imaging system.