Western Blot: Donkey anti-Rabbit IgG, H/L Chains Antibody [DyLight 488] [NBP1-72966] - Lane 1: Rabbit IgG Load: 50 ug per lane Secondary antibody: Rabbit IgG (H&L) Antibody DyLight (TM) 488 conjugated Pre-Adsorbed at 1:1,000 for 60 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa