Flow cytometry / FACS analysis: CD45RC Antibody (C455.1F) [NBP1-28132] - Cells from BALB/c spleen were double stained with rat anti-CD45RC-R-PE and rat anti-mouse CD8a-FITC (Clone 53-6.7), following which small lymphocytes were gated and analyzed on a FACScan? flow cytometer (BDIS, San Jose, CA).