Western Blot: Telomerase reverse transcriptase Antibody [NB600-476] - Figure 1. Immunoblot using anti-hTERT antibody (1:500 dilution). Endogenous levels of mTERT in NIH 3T3 cells (lane 1) and hTERT in HeLa cells (lane 5) are not detectable. After transduction with an hTERT expression vector, both cell types show high levels of hTERT protein (lanes 2 and 6). SY5Y cells, which have high endogenous levels of hTERT, have detectable hTERT protein in both untransduced (lane 3) and transduced (lane 4) cells. The arrow indicates a molecular weight of approximately 127kD, the expected size of hTERT protein.