GTX79225 FACS Image
Flow cytometry analysis of TCR V alpha 2 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with TCR V alpha 2 antibody [F1] (FITC) at a dilution of 1:10 for 60 min at room temperature and re-suspended in PBS for FACS analysis.