GTX629765_41435_IHC_M.jpg
GTX629765_41435_IHC_M_2.jpg
GTX629765_41435_IHC_M_3.jpg
GTX629765_41435_20141114_Dot blot.jpg
 mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-HydroxymethylCytidine antibody [GT13612] (GTX629765) at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA. )
 dilution: 1:100.<BR/>)
 dilution: 1:100.<BR/>)
 dilution: 1:100.<BR/>)
. DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody (GTX629765) at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine)