GTX628887 IP Image
EGFR antibody [GT133] immunoprecipitates EGFR protein in IP experiments.
IP samples: A431 whole cell extract
A. 40 ug A431 whole cell extract
B. Control with 4 ug of preimmune Mouse IgG
C. Immunoprecipitation of EGFR protein by 4 ug EGFR antibody [GT133] (GTX628887)
5 % SDS-PAGE
The immunoprecipitated EGFR protein was detected by EGFR antibody [GT133] (GTX628887) diluted at 1:5000.
[EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent]

GTX628887 WB Image
Non-transfected (?) and transfected (+) A431 whole cell extracts (15 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT133] (GTX628887) diluted at 1:8000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX628887 WB Image
Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT133] (GTX628887) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX628887 ICC/IF Image
EGFR antibody [GT133] detects EGFR protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde for 10 min.
Green: EGFR protein stained by EGFR antibody [GT133] (GTX628887) diluted at 1:300.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

GTX628887 IHC-P Image
EGFR antibody [GT133] detects EGFR protein at cytosol on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
EGFR antibody [GT133] (GTX628887) dilution: 1:500.

GTX628887 IHC-P Image
EGFR antibody [GT133] detects EGFR protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
EGFR antibody [GT133] (GTX628887) dilution: 1:500.