GTX60832 ICC/IF Image
ICC/IF analysis of HeLa cells using HDAC3 antibody (red) and DAPI. Cells were fixed with 4% formaldehyde for 10f and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC3 antibody at a dilution of 1:500.
GTX60832 ChIP Image
ChIP was performed with HeLa extracts and either 5 ug of HDAC3 antibody or control IgG. The precipitated DNA was detected by qPCR with primers targeting to GAPDH promoter, c-fos promoter and the coding region of p21, a known target gene of HDAC3.