GTX54957 WB Image
Western blot analysis of Cyclin D1 (pT286) expression in MCF7 EGF-treated (A); rat muscle (B) whole cell lysates.

GTX54957 IHC-P Image
Immunohistochemical analysis of Cyclin D1 (pT286) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.