GNT graphics

GTX50009 ICC/IF Image
HeLa cells were stained with monoclonal anti-EGLN1 antibody (Green). Nucleus and Actin were stained by Phalloidin-TRITC (Red) or Hoechst 33342 (Blue).

GNT graphics

GTX50009 WB Image
Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human EGLN1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system