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 were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRAS antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HeLa cell lysate
Lane 2. : HepG2 cell lysate
Lane 3. : Ramos cell lysate
Lane 4. : A549 cell lysate
Lane 5. : Balb/3T3 cell lysate )
. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).)
 for nucleus staining and monoclonal anti-human KRAS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)