HeLa cell (human) extract and Human uterine mesodermal tumor cell-SKUT extract were separated on SDS-PAGE and probed with Monoclonal Anti-CDK 4, clone DCS-31 (Cat. No. GTX26315). The antibody was developed with Anti-Mouse IgG (Fab), alkaline phosphatase and a NBT/BCIP substrate.?Antibody dilution 1:2,000.