GTX221666-01 WB Image
Comparison of western blot analysis after immunoprecipitation with traditional secondary antibody (1) and EasyBlot anti-Rabbit IgG kit (GTX225856-01)(2). (A) Input, 20ug of HCT116 lysate. (B) and (C): HCT116 lysates were immunoprecipitated using 2.5 ug of normal rabbit IgG (B) or anti-p53 antibody (GTX102965)(C).? The precipitated protein was detected by GTX102965 diluted at 1:10000.

GTX221666-01 WB Image
GST-tagged protein (Mw: 45 kDa) was immunoprecipitated from E. coli lysate using 8 ug of anti-GST antibody (GTX110736). The precipitated protein was detected by GTX110736 diluted at 1:3000.

A: EasyBlot
B: Goat anti-rabbit antibody

GTX221666-01 WB Image
Comparison of western blot analysis after immunoprecipitation with traditional secondary antibody, EasyBlot anti-Rabbit IgG (HRP) (GTX221666-01) and EasyBlot anti-Rabbit IgG kit (GTX225856-01). A,C,and E: E. coli lysate with overexpressed GST-tagged protein B,D,and F: IP result IP and 1st antibody: anti-GST antibody

GTX221666-01 WB Image
EasyBlot anti Rabbit IgG (HRP) antibody detects native-rabbit IgG by western blot analysis.Recombinant Protein (8 and 0.1 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with EasyBlot anti Rabbit IgG (HRP) antibody (GTX221666-01) at a dilution of 1:5000.