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GTX133435 ICC/IF Image
EGFR (phospho Tyr1148) antibody detects EGFR (phospho Tyr1148) protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EGFR (phospho Tyr1148) protein stained by EGFR (phospho Tyr1148) antibody (GTX133435) diluted at 1:500.
Blue: Hoechst 33342 staining.

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GTX133435 WB Image
Untreated (?) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1148) antibody (GTX133435) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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GTX133435 IHC-P Image
EGFR (phospho Tyr1148) antibody detects EGFR protein at cell membrane in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
EGFR (phospho Tyr1148) antibody (GTX133435) diluted at 1:500.