GTX132170 WB Image
Untreated (?) and treated (+) HCT116 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Chk1 (phospho Ser317) antibody (GTX132170) diluted at 1:500.

GTX132170 ICC/IF Image
Chk1 (phospho Ser317) antibody detects Chk1 (phospho Ser317) protein at nucleus by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 100 J/m2 UVC and recover for 8 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Chk1 (phospho Ser317) protein stained by Chk1 (phospho Ser317) antibody (GTX132170) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.