GTX116011 WB Image
Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX116011 ICC/IF Image
DNMT1 antibody [N1], N-term detects DNMT1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNMT1 protein stained by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:2000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

GTX116011 IP Image
DNMT1 antibody [N1], N-term immunoprecipitates DNMT1 protein in IP experiments.
IP samples: HeLa nuclear extract
A. Control with 4 ug of preimmune Rabbit IgG
B. Immunoprecipitation of DNMT1 protein by 4 ug DNMT1 antibody [N1], N-term (GTX116011)
5 % SDS-PAGE
The immunoprecipitated DNMT1 protein was detected by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX116011 WB Image
Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000.

GTX116011 IHC-P Image
DNMT1 antibody [N1], N-term detects DNMT1 protein at cytoplasm and nucleus in human endometrial cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial cancer.
DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:500.

GTX116011 WB Image
Wild-type (WT) and DNMT1 knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX116011 ChIP assay Image
Cross-linked ChIP was performed with HCT116 chromatin extract and 5 ug of either control rabbit IgG or anti-DNMT1 antibody. The precipitated DNA was detected by PCR with primer set targeting to ILIR2.