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GTX113266 IHC-P Image
ROCK1 antibody [N1N2], N-term detects ROCK1 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:500.

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GTX113266 ICC/IF Image
ROCK1 antibody [N1N2], N-term detects ROCK1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ROCK1 protein stained by ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

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GTX113266 WB Image
Non-transfected (?) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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GTX113266 WB Image
ROCK1 antibody [N1N2], N-term detects ROCK1 protein by western blot analysis.
A. 30 ug NIH-3T3 whole cell extract
B. 30 ug C2Cl2 whole cell extract
5% SDS-PAGE
ROCK1 antibody [N1N2], N-term (GTX113266) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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GTX113266 IP Image
ROCK1 antibody immunoprecipitates ROCK1 protein in IP experiments. IP Sample: A431 whole cell lysate/extract A : Control with 3 ug of pre-immune rabbit IgG B : Immunoprecipitation of ROCK1 by 3 ug of ROCK1 antibody (GTX113266) 5% SDS-PAGE The immunoprecipitated ROCK1 protein was detected by ROCK1 antibody (GTX113266) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

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GTX113266 WB Image
Wild-type (WT) and ROCK1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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GTX113266 WB Image
ROCK1 antibody [N1N2], N-term detects ROCK1 protein by western blot analysis.
A. 50 ug rat brain lysate/extract
5% SDS-PAGE
ROCK1 antibody [N1N2], N-term (GTX113266) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GNT graphics

GTX113266 WB Image
Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.