Nuclear extract (50 g) from human HEK293, human melanoma (G361), mouse wildtype embryonic fibroblasts (+/+) or mouse H2AX knockout embryonic fibroblasts (-/-). Antibody: GTX11174 used at 0.1g/ml. Detection: Chemiluminescence with 30 second exposure.

Detection of gamma-H2AX by Immunofluorescence. Samples: Wildtype (H2AX +/+) or H2AX knockout (H2AX -/-) mouse embryonic fibroblasts. Antibody: Affinity purified GTX11174 used at 2ug/ml. Detection: Rhodamine red labelled goat anti-rabbit IgG.