Human uterine mesodermal tumor cell extract was separated on SDS-PAGE and probed with Monoclonal AB to Cyclin D1 (DCS-6) (GTX10540). The antibody was developed with Anti-Mouse IgG (Fab specific), Alkaline Phosphatase conjugate and a NBT/BCIP substrate. 
Lanes 
1. Antibody diluted 1/200
2. Antibody diluted 1/400