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GTX103232 IP Image
Immunoprecipitation of GRP94 protein from HeLa whole cell extracts using 5 ug of GRP94 antibody (GTX103232).
Western blot analysis was performed using GRP94 antibody (GTX103232).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

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GTX103232 WB Image
GRP94 antibody detects GRP94 protein by Western blot analysis. Whole cell extracts (30 ug) was separated by 7.5 % SDS-PAGE, and blotted with GRP94 antibody (GTX103232) diluted by 1:1000

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GTX103232 ICC/IF Image
GRP94 antibody detects GRP94 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GRP94 protein stained by GRP94 antibody (GTX103232) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

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GTX103232 WB Image
Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP94 antibody (GTX103232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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GTX103232 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GRP94(GTX103232) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (red) at 1:2500.

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GTX103232 IHC-P Image
Immunohistochemical analysis of paraffin-embedded HEP3B xenograft, using GRP94(GTX103232) antibody at 1:100 dilution.

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GTX103232 WB Image
Wild-type (WT) and GRP94 knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP94 antibody (GTX103232) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GNT graphics

GTX103232 WB Image
GRP94 antibody detects GRP94 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5 % SDS-PAGE, and blotted with GRP94 antibody (GTX103232) diluted by 1:1000

GNT graphics

GTX103232 IHC-P Image
GRP94 antibody detects GRP94 protein at cytoplasm in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
GRP94 antibody (GTX103232) diluted at 1:500.