GTX101497 ICC/IF Image
Oct4 antibody detects Oct4 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Oct4 protein stained by Oct4 antibody (GTX101497) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

GTX101497 WB Image
Oct4 antibody detects Oct4 protein by western blot analysis.
A. 30 ug human ESC whole cell extract
12% SDS-PAGE
Oct4 antibody (GTX101497) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101497 WB Image
Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX101497) diluted at 1:100000.

GTX101497 FACS Image
Flow cytometry on human embryonic stem cells, staining with Oct4 (GTX101497)antibody at 1:100 dilution(purple) or rabbit IgG (black).

GTX101497 ICC/IF Image
Immunofluorescence analysis of hESCs on CF1 feeders, using OCT3/4(GTX101497) antibody at 1:200 dilution.

GTX101497 IHC-P Image
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using OCT3/4(GTX101497) antibody at 1:500 dilution.

GTX101497 IP Image
Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of Oct4 by 2 ug of Oct4 antibody (GTX101497) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX101497) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101497 WB Image
Sample (20 ug of whole cell lysate)
A: mouse ESC
12% SDS PAGE
GTX101497 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101497 ICC/IF Image
Immunofluorescence analysis of mESCs on CF1 feeders, using OCT3/4(GTX101497) antibody at 1:200 dilution.