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GTX100659 WB Image
Non-transfected (?) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (GTX100659) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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GTX100659 ICC/IF Image
SOD1 antibody detects SOD1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SOD1 protein stained by SOD1 antibody (GTX100659) diluted at 1:100.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

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GTX100659 WB Image
Western blot analysis of SOD1 (GTX100659, upper panel) and beta-actin (GTX109639, lower panel)?
Sample (30 ug of whole cell lysate)?
A: HeLa mock control?
B: HeLa transfected shSOD1
15% SDS PAGE?
GTX100659 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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GTX100659 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SOD1(GTX100659) antibody at 1:500 dilution.

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GTX100659 IHC-P Image
Immunohistochemical analysis of paraffin-embedded mouse muscle, using SOD1(GTX100659) antibody at 1:500 dilution.

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GTX100659 IHC-P Image
Immunohistochemical analysis of paraffin-embedded rat brain, using SOD1(GTX100659) antibody at 1:500 dilution.

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GTX100659 WB Image
SOD1 antibody detects SOD1 protein by western blot analysis.
A.30 ug NT2D1 whole cell lysate/extract
B.30 ug PC-3 whole cell lysate/extract
C.30 ug U87-MG whole cell lysate/extract
D.30 ug SK-N-SH whole cell lysate/extract
15% SDS-PAGE
SOD1 antibody (GTX100659) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GNT graphics

GTX100659 WB Image
Sample (50 ug of whole cell lysate)
A: Rat brain
15% SDS PAGE
GTX100659 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GNT graphics

GTX100659 WB Image
SOD1 antibody detects SOD1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (GTX100659) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.