EB05865 (3.8g/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05865 (1g/ml) staining of Human Heart lysate (35g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing ILPIP (RC203432) and probed with EB05865 (mock transfection in first lane), tested by Origene.