Whole rat brain lysate with mouse anti-MAP2 antibody. The antibody recognizes the ~280 kDa protein. Right: Mixed neuron/glia cultures stained with mouse anti-MAP2 (green) and also rabbit antibody to neurofilament H (Catalog Number R-1388-50) (red). Since the NF-H protein is largely expressed in neuronal axons, while the MAP2 is only found in neuronal dendrites and perikarya, there is little overlap between these two staining patterns. DNA stain shows nuclei of neurons and non-neuronal cells (blue).