Western blots of Guinea pig brain stem inferior colliculi samples homogenized in Phospho-Sure™ RTD™ (B-100-PS) or Lysis buffer and kept on ice for up to 120 min. Aliquots of the homogenates (30µg) were run on SDS-PAGE and proteins transferred to blotting membrane. Blots were stripped and incubated with 3 antibodies: CREB-P, SAP/JNK-P and ERK 1/2. Image courtesy of Suneja et al (2006).