Detection of Human RANK by Western Blot and Immunoprecipitation. Samples:  Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells.   Antibodies: Affinity purified rabbit anti-RANK antibody A303-807A used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate.  RANK was also immunoprecipitated by rabbit anti-RANK antibody A303-808A, which recognizes a downstream epitope.    Detection: Chemiluminescence with exposure times of 10 seconds (A) and 30 seconds (B).