Detection of Human NDRG3 by Western Blot of Immunoprecipitates. Samples:  Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.  Antibodies: Affinity purified rabbit anti-NDRG3 antibody A303-748A used for IP at 6 ug/mg lysate.  NDRG3 was also immunoprecipitated by rabbit anti-NDRG3 antibody A303-749A, which recognizes a downstream epitope.  For blotting immunoprecipitated NDRG3, A303-749A was used at 1 ug/ml.  Detection: Chemiluminescence with an exposure time of 10 seconds.