Detection of Human Lamin-A and Lamin-C by Western Blot and Immunoprecipitation. Samples :  Whole cell lysate (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells.   Antibodies : Affinity purified rabbit anti-Lamin-A/C antibody A303-430A used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate.  Lamin-A was also immunoprecipitated by rabbit anti-Lamin-A antibody A303-433A, which recognizes a downstream epitope.   Detection: Chemiluminescence with exposure times of 1 second (A and B).